Bunnie Butler
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Sertraline HCL ( Zoloft )decreased significantly the rate of 1-N- and 3-N-demethylation and 8-hydroxylation (Ki 37.3, 69.3 and 64 microM, online pharmacy respectively), drugstore discount pharmacy while its effect on 7-N-demethylation of caffeine was less the veriest (Ki 92.1 microM). The obtained results provide further online pharmacy indirect evidence that apart from CYP1A2, other CYP isoforms are also important for the metabolism of caffeine.. oral contraceptives contraceptive Nefazodone displayed clear effect on 3-N- and 7-N-demethylation plan-b (Ki 68.8 and 66.4 microM, respectively), but was weak in inhibiting 1-N-demethylation and 8-hydroxylation of caffeine online propecia finasteride medication (Ki 110 and 186 microM, respectively). In summary, we have observed intra- and inter-drug differences in the remeron inhibitory effects of the antidepressants on the four oxidation pathways of caffeine in rat liver microsomes. Dixon analysis of caffeine metabolism hair removal carried out in the control liver microsomes, in the absence and presence of the antidepressant drugs sho that desipramine and clomipramine exerted the most potent alesse inhibitory effect on caffeine metabolism. In vitro study.Caffeine undergoes 3-N-demethylation via CYP1A2, as well as 1-N-demethylation, 7-N-demethylation and 8-hydroxylation, which may involve antibiotics other CYP isoenzymes. Its effect on 7-N-demethylation was rather weak (Ki 97.8 microM). Desipramine decreased the rates of 1-N-, 3-N- and 7-N-demethylations, and 8-hydroxylation cialis of caffeine (Ki 23.3, 36.6, 23.3 and 63.3 microM, respectively), the effect on 1-N- and 7-N-demethylation being the most pronounced. In contrast to the above-tested antidepressants, Mirtazapine ( Remeron ) did not decrease significantly the oxidation rates of 3-N-demethylation or 8-hydroxylation (Ki 264 and 455 microM, respectively) and had no effect on other oxidation pathways of caffeine. Effects of classic and newer antidepressants on the oxidation pathways of caffeine in rat liver. Clomipramine sho distinct inibition of 1-N- and 3-N-demethylation and 8-hydroxylation of caffeine, the effects on N-demethylations being the most pronounced (Ki 38.6, 34.8, 45.6 microM, respectively). The obtained results sho that all the investigated antidepressants, with an exception of Mirtazapine ( Remeron ), added in vitro to liver microsomes had an inhibitory effect on caffeine metabolism (via competitive or mixed mechanism), though their potency towards particular metabolic pathways was different. The aim of the present study was to investigate the influence of clomipramine, desipramine, Sertraline HCL ( Zoloft ), nefazodone and Mirtazapine ( Remeron ) on cytochrome P-450 activity measured by caffeine oxidation in rat liver microsomes. The tested antidepressants (with an exception of Mirtazapine ( Remeron )) may lead to drug-drug metabolic interactions at a level of a few CYP isoforms.
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